Figure 5.

Current/voltage relationship of HvCNGC2-3-expressing oocytes in a 1:4 mixture of Na⁺ and K⁺ media. Currents were recorded by the two-electrode voltage-clamp technique in a bathing solution containing 19.2 mM NaCl and 76.8 mM KCl in the absence of a pre-incubation with cyclic nucleoside monophosphates (cNMP-free, circles), and in the presence of a pre-incubation with 10 µM 8Br-cGMP (triangles) or 8Br-cAMP (squares) for 30 min. (A) representative raw recording traces; (B) current/voltage curve. Error bars indicate standard deviation (n = 9, 13 and 6 for cNMP-free, cGMP and cAMP, respectively). I, membrane current. Vm, membrane potential.