Table 2.
Oxford nanopore technologies MinION sequencing yields and basic details and breakdown of centrifuge classification
Total | Mean | Median | Low complexity | Human | Bacteria | |||||
---|---|---|---|---|---|---|---|---|---|---|
Sample | Bases | Reads | read length | read length | bases | reads | bases | reads | bases | reads |
229a | 204,346,556 | 124,218 | 1645.06 | 1745 | 113,836 | 69 | 198,972,861 | 117,821 | 1,692,097 | 914 |
249a | 723,925,562 | 585,098 | 1237.27 | 1006 | 403,668 | 370 | 563,888,189 | 411,612 | 44,502,912 | 34,773 |
259a | 1,057,865,247 | 600,291 | 1762.25 | 1321 | 390,209 | 289 | 949,663,786 | 502,426 | 512,827 | 312 |
312a | 1,121,119,742 | 1,004,818 | 1115.74 | 674 | 1,905,423 | 1044 | 1,038,235,876 | 882,763 | 30,426,198 | 14,948 |
335a | 2,847,687,425 | 1,717,810 | 1657.74 | 1171 | 2,835,054 | 1362 | 2,783,128,118 | 1,605,466 | 1,388,748 | 989 |
352a | 803,638,340 | 986,867 | 814.33 | 609 | 567,656 | 630 | 669,796,136 | 752,022 | 459,779 | 579 |
354a | 706,380,170 | 945,929 | 746.76 | 596 | 680,560 | 848 | 570,485,740 | 717,662 | 2,151,551 | 2443 |
509a | 2,740,060,527 | 4,940,241 | 554.64 | 439 | 16,355,839 | 24,413 | 1,199,779,866 | 1,352,438 | 6,240,425 | 2628 |
506a | 2,451,399,949 | 4,700,013 | 521.57 | 431 | 20,014,343 | 23,631 | 1,161,796,584 | 1,671,726 | 4,705,919 | 2139 |
Bacteria, Human with a centrifuge score greater than 150, and total reads including unclassified reads. Samples 509a and 506a are culture negatives and used as negative controls. Results are after removing low complexity reads