Figure 4.

SP converts naïve CD11c⁺ cells into CS cells. (A) Adoptive transfer of in vitro generated CS cells was tested by incubating naïve CD11c⁺ BALB/c spleen cells with either SP (1 pg/mL) or PBS for 1 hour prior to IV injection into naïve BALB/c mice. ACAID was induced by AC injection of OVA, and ear swelling was assessed 10 days after SC immunization with OVA plus CFA. (B) The capacity of in vitro generated CD11c⁺ CS cells to disable Treg activity in situ was determined using a LAT assay. Naïve CD1c⁺ cells were incubated with either SP (1.0 pg/mL) or PBS for 1 hourr, washed in PBS, and added to a cell suspension containing ACAID Tregs, immune cells, and OVA-pulsed APC, which were injected into the ears of naïve mice and tested in a conventional LAT assay. Results are expressed as mean ± SEM. This experiment was performed twice with similar results. There were five mice in each group.