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. 2018 Sep 27;18:263. doi: 10.1186/s12906-018-2320-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of ES on the MAPK signaling pathway in P. acnes-indcued THP-1 cells. THP-1 cells were pre-treated with 0.1, 1 and 10 μg/ml of ES for 1 hrs incubation and then stimulated with P. acnes for 1 hrs incubation. (a) Western blot analysis shows that phosphorylation of p38, ERK and JNK is suppressed by ES treatment. (b) MAPKs/β-actin ratio were determined by densitometry. Values represent mean ± S.E.M. Data were analyzed by Tukey post hoc test ( *P < 0.05 versus non-treatment and **P < 0.05 versus P.acnes alone)