Fig. 5.

ERK activation is required for autophagy suppression by MTE treatment. A549 or H1975 cells were treated with MTE at concentration of 0, 20, 40, and 80 mg/ml for 24 h in the absence or presence of U0126. a, b The protein levels of ERK, p-ERK in treated cells determined by Western blot in A549 (a) and H1975 (b) cells. The ratio of p-ERK vs ERK protein levels in treated cells was counted. c, d The protein levels of LC3-II, p62, LAMP1 and Cathepsin B in treated cells were determined by Western blot in A549 (c) and H1975 (d) cells