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. 2018 Jun 4;4:9. doi: 10.1038/s41378-018-0012-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — SEM images of membrane top surfaces after assays: a–c after a positive assay with 1 nM PLP; d after a positive assay with 50 fM PLP; e after a control assay with zero PLP concentration in which AuNPs incubation was omitted; f after a control assay with zero PLP concentration; and g after a control assay with 1 nM PLP concentration in which DNA stretching was omitted. In a–c, full AuNWs are visible; in a–d, gold rims and gold bumps are visible; in c, a fragmented AuNW is visible; in b–d, f–g, AuNPs are visible; in f, the arrow indicates a gold evaporation shadow. AuNPs, gold nanoparticles; AuNWs, gold Nanowires; PLP, padlock probes; SEM, scanning electron microscopy