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. 2018 Sep 21;12:313. doi: 10.3389/fncel.2018.00313

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Copyright © 2018 Carrillo-Jimenez, Puigdellívol, Vilalta, Venero, Brown, StGeorge-Hyslop and Burguillos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Small interfering RNA (siRNA) delivery and efficiency transfection analysis using the Glial-Mag technology. (A,B) Assessment of the delivery of siGLO reagent through flow cytometry represented by dot plots and histogram into mouse primary cortical microglia cells using increasing amounts of Glial-Mag. Analysis of triggering receptor expressed in myeloid cells 2 (TREM2; C) and CD33 (D) gene expression after transfection with specific siRNA measured by room-temperature (RT)-qPCR. Results are presented as mean (B–D) ± SD (B,C). Data are from one (A,B) or five (C) or three (D) independent experiments. **P < 0.01 and ***P < 0.001. All analyses were performed using two-tailed Welch’s t-test.