Figure 2.

Effect of Glial-Mag technology over cell survival and glia proliferation upon 48 h transfection in primary cortical microglial and cerebellar granule cells (CGCs) primary cultures in wild type mouse and rats. (A) Representative images and insets showing microglia (IB4, green), PI (as a necrotic marker, red) and nuclei (Hoechst, blue) staining in cortical primary cultures from wild type mouse and rat transfected with or without siRNA non-targeting (siNT). (B) Quantitative analysis showing microglial density in mouse and rat cortical primary cultures transfected with or without siNT. (C,D) Quantitative analysis showing neuronal (C) and microglia and astrocyte density (D) in mouse and rat CGC primary cultures transfected with or without siNT. (E) Analysis of IL-6 release into the media in naïve and 48 h transfected siNT treated cells ± LPS treatment (8 h; 10 ng/ml). Results are presented as mean ± SEM (B–D) and ± SD (E). Quantitative analysis of cell numbers in (B–D) represent four microscopic fields (mouse) or four microscopic fields in duplicate or triplicate (rat) for each condition from three independent experiments for both mouse and rat. Data are from five (for naïve and siNT) and for three (naïve + LPS and siNT + LPS) independent experiments in (E). All analyses were performed using one-way ANOVA and Tukey’s multiple comparisons post hoc test. n.s stands for non-significant. *P < 0.05 and **P < 0.01 compared to naïve condition. Scale bar, 50 μm.