Figure 5.

3-T₁AM induces Ca²⁺ influx in GT1-7, N39 and N41 cell lines. Changes in cytosolic free Ca²⁺ are depicted as the ratio of the fluorescence induced by the excitation wavelength at 340 and 380 nm. 10 μM 3-T₁AM induces an increase on intracellular Ca²⁺ concentration in (A) GT1-7 (n = 10), (B) N39 (n = 15) and (C) N41 (n = 15) cells. Notably, 10 μM 3-T₁AM induces a significantly larger increase in intracellular Ca²⁺ in N41 cell line. Without compound application, no changes in Ca²⁺ influx could be observed (n = 10). Compounds were added to cells at the time points indicated by the arrow and n indicates number of the single cells. Experiments were performed in 400 s. The total number of cells was collected in five independent experiments. Values represent mean ± SEM.