Table 2.
Current properties of the different CaV1.2 mutant and control constructs. Numerical values represent mean ± SEM. Statistical differences between mutants and controls were determined with one-way ANOVA (Ipeak: F3, 56 = 5.3; p = 0.0027; V1/2: F3,56 = 6.3; p = 0.0009), followed by a Holm-Sidak multiple comparison test. Differences against control are represented as *(P-value), difference against CaV1.2-D4N are represented as †(P-value) and difference against CaV1.2-ΔE33 are shown as ‡(P-value). Mean differences and their associated adjusted P-values for each of the six comparisons tested with the Holm-Sidak multiple comparison test.
| Ipeak (pA/pF) | Significance (P value) | V1/2 (mV) | Significance (P value) | Vrev (mV) | kact (mV) | Gmax (nS/nF) | Tau (ms) | Ires200/Ipeak | n | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CaV1.2 | −13.8 ± 1.7 | 22.0 ± 1.7 | 93.48 ± 2.2 | 7.3 ± 0.4 | 275.5 ± 30.4 | 2.5 ± 0.29 | 0.62 ± 0.03 | 21 | |||||
| CaV1.2-D4N | −7.9 ± 1.7 | *(0.0393) | 27.1 ± 2.3 | 95.5 ± 2.9 | 8.9 ± 0.4 | 169.2 ± 28.9 | 2.5 ± 0.18 | 0.60 ± 0.03 | 14 | ||||
| CaV1.2-ΔE33 | −14.9 ± 1.9 | †(0.0393) | 15.7 ± 1.4 | †(0.0005) | 88.25 ± 1.8 | 7.0 ± 0.4 | 282.8 ± 29.8 | 2.1 ± 0.31 | 0.61 ± 0.04 | 12 | |||
| CaV1.2-ΔE33-D4N |
−6.9 ± 1.1 |
*(0.0233), ‡(0.0233) |
19.8 ± 1.1 |
†(0.0379) |
85.79 ± 2.1 |
7.7 ± 0.3 |
152.2 ± 21.2 |
2.6 ± 0.21 |
0.55 ± 0.04 |
13 |
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| |
Ipeak |
V1/2 |
|||||||||||
| |
Mean Difference |
Adjusted P Value |
Mean Difference |
Adjusted P Value |
|||||||||
| CaV1.2 vs. CaV1.2-D4N | −5.878 | 0.0393 | −5.073 | 0.1032 | |||||||||
| CaV1.2 vs. CaV1.2-ΔE33 | 1.025 | 0.8901 | 6.331 | 0.0515 | |||||||||
| CaV1.2 vs. CaV1.2-ΔE33-D4N | −6.898 | 0.0233 | 2.199 | 0.3655 | |||||||||
| CaV1.2D4N vs. CaV1.2ΔE33 | 6.903 | 0.0393 | 11.4 | 0.0005 | |||||||||
| CaV1.2D4N vs. CaV1.2ΔE33-D4N | −1.02 | 0.8901 | 7.271 | 0.0379 | |||||||||
| CaV1.2ΔE33 vs. CaV1.2ΔE33-D4N | −7.923 | 0.0233 | −4.133 | 0.2539 | |||||||||