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. 2018 Sep 21;25(1):1740–1745. doi: 10.1080/10717544.2018.1510065

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Evaluation of transfection efficiency by the combination of 25 kDa PEI and LMW PEI (800 Da PEI or 1.8 kDa PEI). From left to right, cells without any treatment is used as the negative control, cells are transfected by 0.5 μg 25 kDa PEI +1 μg pGFP, 50 μg 800 Da PEI +1 μg pGFP, 50 μg 1.8 kDa PEI +1 μg pGFP, 0.5 μg 25 kDa PEI +1 μg pGFP +50 μg 800 Da PEI and 0.5 μg 25 kDa PEI +1 μg pGFP +50 μg 1.8 kDa PEI, respectively. Gene transfection efficiency of each group is quantified by flow cytometry. Each column represents the mean ± S.D. (n = 3). *p < .05 versus other groups.