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Summary of Procedures for Trans-omic Network Construction
(A) Induced and basal insulin secretion in vivo (Polonsky et al., 1988).
(B) The trans-omic network was constructed through Steps I to III by integrating transcriptome, protein (western blotting), and metabolome data. The detailed procedures can be found in Methods.
(C) Definition of EC50, an index of sensitivity to insulin doses (left), and T1/2, an index of time constant (right).
