Figure 4.
Step III: Connection of the IRMs and the IRGs of Metabolic Enzymes
(A) Distributions of EC50 (left) and T1/2 (right) values estimated for the increased IRMs (red bars) and the decreased IRMs (blue bars). To identify a high confidence set of IRMs from multiple experimental datasets, we performed a 3-way ANOVA with the insulin doses, stimulation times, and data acquired on different days as main factors (see Methods).
(B) IRMs projected onto the KEGG metabolic pathways. Arrows indicate whether an IRM increased or decreased by insulin stimulation. The colors of the outline and the labels indicate the classes classified by the EC50 and T1/2 values of the IRMs: dark blue, Class 1; cyan, Class 2; red, Class 3; pink, Class 4.
(C) Metabolites in the central carbon metabolism. The black frames indicate the metabolites that did not show significant changes in response to insulin in a 3-way ANOVA, and gray frames indicate those with unmeasured points at one and more time points (NaN). The other colors correspond to the classes classified by the EC50 and T1/2 values of IRMs.
(D) The IRGs of metabolic enzymes predicted to regulate the indicated IRMs. The colors of the IRGs and IRMs indicate the classes classified by the EC50 and T1/2 values.