Figure 1.

Conservation of the hnRNP B1-specific exon across species.

(a) Model of protein domains of hnRNP A2 and B1, with B1-specific domain highlighted. (b) Multiple sequence analysis of hnRNP B1 genomic sequence in human, mouse, rat, cow, elephant, and opossum reference sequences, with B1-specific exon and next downstream exon highlighted, demonstrating high degree of conservation of the B1-specific exon in all eutherian species. Mismatches from human sequence are colored in red. (c) Immunoprecipitation using antibody specific to hnRNP B1 specifically retrieves hnRNP B1 in both human and mouse samples. (d) RT-PCR primers surrounding B1-specific exon 2 demonstrate inclusion of B1 exon in total RNA from both human MCF7 and mouse C2C12 cells.