Figure 6.

Model of hnRNP B1 interaction with HOTAIR.

(a) hnRNP B1 on chromatin can bind to transcripts co-transcriptionally, or bind to lncRNAs associating with other loci in trans.(b) On the lncRNA HOTAIR, hnRNP B1 preferentially binds to intron 3, which bisects an RNA-RNA interaction hotspot. Retention of intron 3 may not disrupt the ability of HOTAIR to pair with other RNAs in the hotspot region. Following splicing of intron 3, B1 remains bound to nearby regions of HOTAIR, continuing to promote RNA-RNA interactions from the hotspot region.