Figure 1.

Identification of NRDE-2-interacting proteins by co-IP and mass spectrometry.

(a) Silver staining of FLAG co-IPs from control and NRDE-2-3FLAG stable HEK293 cell lines used for mass spectrometry. (b) Fractions from Superose 6 gel filtration of 3FLAG-Mtr4-expressing HEK293 cell lysates treated with Benzonase/RNase A were analyzed by WB, using antibodies against proteins shown on the right. Approximate molecular sizes are indicated at the top and fractions pooled at the bottom. The asterisk indicates a non-specific band. (c) The protein-protein interaction network among the proteins shared in NRDE-2-3FLAG and 3FLAG-Mtr4 co-IP that are absent in HEK293 control co-IP was constructed using STRING v10.5 (http://string-db.org) with the high confidence setting. Disconnected nodes are not shown in the network.