Figure 4.

R-loop levels at RPL13A, BACT and EGR1 are not affected by NRDE-2 or Mtr4 KD.

(a) DRIP assays were performed in HeLa cell after 72 h of siRNAs transfection. DRIP signal was also measured after RNase H1 treatment (right). (b) DRIP signal was normalized to the signal in non-transfected cell. Error bars represent the average of two different experiments. SD shown. (c) NRDE-2 and Mtr4 KDs confirmation by WB. None = no transfection, NC: siRNA control transfection. (D) NRDE-2 ChIP at various loci. ChIP assays were performed in HeLa cell after 72 h of siRNAs transfection. n = 2