Table 1.
Adapter dilutions and number of PCR cycles for sRNA-seq library preparation.
| BSC |
CLT |
NEB |
QIA |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ID | Sample | Input (ng) | AD | PCR | AD | PCR | AD | PCR | AD | PCR |
| U1 | miRXplore 1 | 5 | 1/1.3 | 16 | N/A | 13 | none | 15 | none | 15 |
| U2 | miRXplore 2 | 5 | 1/1.3 | 16 | 13 | none | 15 | none | 15 | |
| P1 | hsa plasma 1 | 5 | 1/4 | 23 | 16 | 1/4 | 18 | 1/5 | 22 | |
| P2 | hsa plasma 2 | 5 | 1/4 | 23 | 16 | 1/4 | 18 | 1/5 | 22 | |
| S1 | hsa serum 1 | 5 | 1/4 | 23 | 16 | 1/4 | 18 | 1/5 | 22 | |
| S2 | hsa serum 2 | 5 | 1/4 | 23 | 16 | 1/4 | 18 | 1/5 | 22 | |
| B1 | mmu brain 1 | 200 | 1/1.3 | 16 | 13 | none | 15 | none | 15 | |
| B2 | mmu brain 2 | 200 | 1/1.3 | 16 | 13 | none | 15 | none | 15 | |
Abbreviations: miRXplore Universal Reference (U), human plasma (P), human serum (S), mouse brain (B), adapter dilution (AD).