Figure 2.

Flnb pre- and mRNA are differentially edited in mouse brain. A) Editing levels at the Q/R site of Flnb pre- and mRNA were measured by Sanger sequencing in two biological replicas of wild type olfactory bulb, cortex and cerebellum. In all samples unspliced Flnb pre-mRNA is highly edited, while only 30% to 50% of spliced Flnb mRNAs are edited. B) Chromatrograms showing Flnb pre-mRNA (top panel) and Flnb mRNA (bottom panel) in wild type cerebral cortex. Arrows point the S/G and Q/R editing sites in Flnb exon and the adenosine edited in the downstream intron.