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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: J Am Chem Soc. 2018 Jun 15;140(25):7764–7768. doi: 10.1021/jacs.8b01989

Figure 3.

Figure 3.

Simultaneous determination of the synaptic cholinergic transmitter concentration dynamics and the number of transmitter molecules (N) during discrete synaptic release events (A). NA is Avogadro’s number, Q is the charge based on integration of amperometric current peak, z is the charge of the transmitter molecule (equals 1 for acetylcholine), and F is Faraday’s constant. Synaptic transmitter concentration profile was obtained from amperometric peak based on current expression at the nanoelectrode, c=i4xzFDa (Supporting Information). (B) Number of neurotransmitter molecules and number of moles released during singlet (N = 8), doublet (N = 4) and multiplet events (N = 4) measured from the single synapse shown in Fig. 1B in response to 6 repetitive chemical stimulations. Release events were constantly observed during each of six chemical stimulations. A variation in synaptic cholinergic transmitter concentration was observed, which is likely due to variation in synaptic vesicles sizes as observed by TEM52.