Figure 3. CAPNS1 protein and calpain-1/2 activities are ablated by capns1 deletion.

(Upper) Lysates from capns1^flox/flox MTECs transduced with Cre-expressing (KO) or control (WT) retroviruses; or capns1^+/+ (WT) or capns1^−/− (KO) mouse embryonic fibroblasts (MEFs) were subjected to immunoblotting analysis with antibodies recognizing CAPNS1 or tubulin. (Lower) Casein zymography assessment of calpain-1 and calpain-2 activities in lysates from the same panels of MTEC and MEF cells.