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. 2018 Sep 7;9(70):33382–33395. doi: 10.18632/oncotarget.26078

Figure 4. capns1 KO is associated with attenuated MTEC in vitro invasion but no effect on migration.

Figure 4

(A) Temporal profiles of capns1 KO and WT MTEC migration behavior in scratch-wound healing assays. Fifteen thousand MTECs were seeded on ImageLock 96-well plates and confluent monolayers were scratch-wounded after an overnight incubation. Migration was monitored in an IncuCyte system for 24 h. Data are the means ± SD of three independent experiments run in sextuplicate. (B) Boyden chamber assays measured capns1 KO and WT MTEC invasion after 24 h through 8 μm pores of transwells coated with 15 μg of matrigel. Data are means ± SD of two independent experiments run in triplicate (p = 0.006, student's t-test).