Figure 6. capns1 KO is associated with reduced MTEC tumorigenic potential.

One × 10⁶ capns1 KO or WT MTECs were engrafted into the number four mammary glands of female Rag2^−/− IL-2Rgc^−/− mice (n = 6 for each cohort). (A) Caliper measurements of tumor volumes. Median time to onset of palpable tumors at the engraftment site were 79 or 29 days for KO and WT, respectively (p = 0.0009^***, Log-rank (Mantel-Cox) test). (B) Late arising tumors in mice engrafted with capns1 KO MTECs display CAPNS1 expression. Immunoblot analysis of tumor lysates reveals CAPNS1 expression in tumors from both capns1 WT and KO engrafted MTECs. Lysates from WT and KO MEF cells served as positive and negative controls for CAPNS1. Immunoblotting for tubulin confirmed comparable sample loading.