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. 2018 Sep 7;9(70):33249–33257. doi: 10.18632/oncotarget.26064

Figure 3. D- Arg Peptide is more effective than the L- Arg Peptide in (A) DU145 cells and (B) H196 SCLC cells at 24 hours of treatment.

Figure 3

In this assay, 5000 cells per well were plated for the 24-hour time point in a 96 well plate on day zero in RPMI media containing 10% FBS. After 24 hours either the D- Arg peptide or the L- Arg Peptide was added in serial dilutions across the plate. Cell viability was assessed at 24 hours of treatment by measuring the absorption at 490nm using the MTS tetrazolium Promega CellTitre 96® Aqueous One Solution Cell proliferation assay according to the manufacturer’s instructions. Each time point was done in triplicate and values are represented by mean with standard mean deviation.