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. 2018 Sep 7;9(70):33249–33257. doi: 10.18632/oncotarget.26064

Figure 4.

Figure 4

A. D- Arg Peptide incubated in 10% FBS RPMI media at 37°C for 24 hours is resistant to proteolysis; it is as effective as the non-incubated D- Arg Peptide in DU145 cells. D- Arg Peptide was incubated in RPMI media containing 10% FBS at 37°C for 24 hours. DU145 cells were seeded in a 96 well plate at 5000 cells in each well. After 24 hours either the incubated D- Arg Peptide or the non-incubated D- Arg Peptide was added to the cells. B. D- Arg and L-Arg Peptide incubated in 10% FBS RPMI media at 37°C for 24 hours. DU145 cells were seeded in a 96 well plate at 5000 cells in each well. After 24 hours either the incubated D- Arg Peptide or the L- Arg Peptide was added to the cells. The cell viability in both experiments were assessed after another 24 hours by measuring the absorption at 490nm using the MTS tetrazolium Promega CellTitre 96® Aqueous One Solution Cell proliferation assay according to the manufacturer’s instructions. Each time point was done in triplicate and values are represented by mean with standard mean deviation.