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. 2018 Sep 28;13(9):e0200617. doi: 10.1371/journal.pone.0200617

Table 2. Information on the major markers in chromosome 4 used to analyze the polymorphisms.

Marker Number Primer Sequence position Digest(s)
CAPS01 F—AGTTCAATGACCAAAGGTGAACT
R—AATGTCACGTTGTCATGTCCA
Chr4:59846 BglII(a/gatct):445+206,651
CAPS02 F -TGCAACTACAGGTGTAAGGCA
R—CCAACAACCATTCACGCAGG
Chr4:67981 SalI(g/tcgac):445+317,762
CAPS03 F—ACCTTGGGAGCCCTTAGAGA
R—AGGTAGAACACTCAAACTTAGGGT
Chr4:137311 HapII(c/cgg):335+316,651
CAPS04 F—GAACGTCCCAAGGAACCACA
R—TCCAACTTCAAACCCTTCCTCT
Chr4:840557 TaqI(t/cga):372+352,724
CAPS05 F—CGTACGTATACCACCACCCG
R—TGGTCATCGTCATCGCTAAGG
Chr4:909302 EcoRI(g/aattc):405+353,758
CAPS06 F—TGACCTATTCTCGCTTGCCC
R—TCCCCTTGCATTTTCCCTCG
Chr4:999594 Eco32I(gat/atc):437+292,729
CAPS07 F—AATAGCTCTTCGTTCATGTACTCT
R—AGACATCACGTGGAAATTAGAAG
Chrr4:3362878 HapII(c/cgg):330+328,658
CAPS08 F—ACCCTACCATGTCTATTAACCATCC
R—AGGAAACAATTGTATAGCTATGGTTT
Chr4:3848297 HindIII(a/agctt):471+252,723
CAPS09 F—AGTGTTCAAATTAACCAAGGGGA
R—ACTCGCATGTCACCTACACG
Chr4:3874492 HindIII(a/agctt):458+252,710
CAPS10 F—TGACTACCAATCAGACAAATCTTGA
R—GGTGGTCAGTGGGAATTGCA
Chr4:4156897 TaqI(t/cga):366+312,678
CAPS11 F—ACATCATCTCTCCCGCTCCA
R—TGTTGCGTTTAGGGTTGGTT
Chr4:4994766 AluI(ag/ct):473+179,652
CAPS12 F—ACCGCTAAAAACTAAATGAGTGTT
R—AGCATGCAAAATAACGCCCG
Chr4:5533196 AsuII(tt/cgaa):714,357+357
CAPS13 F—ATCAATTATGCCTCAATAGGTTTCTT
R—TGGAGTATTGCATTGAGTAGCA
Chr4:6486343 BfaI(c/tag):494+186,680
CAPS14 F—TTCCTTGCAGCCAACTCACT
R—CAAGGGTGCTCGAGAAGGTT
Chr4:6838095 EcoRI(g/aattc):782,492+290
CAPS15 F—TCCTTCAACAGTTGGCGACT
R—CGCCTTGAGTGGGCTATAGG
Chr4:6862096 AluI(ag/ct):408+281,689
SNP02 F—TGCATGATGAGCCTTCTTTGAA
R—TAGACGGGGCTCACAAGTCA
Chr4:48456
SNP03 F—AGAATCAACATGAGAATGCAACA
R—ATCAAAGGACGAGCCCTCAC
Chr4:37932
SSR01 F—GCATATCTAATCTATGAGCACCTAC
R—ACAATATTTTGTTGTAGAGTAGAGGA
Chr4:74647–75150
SSR02 F—GGGTTTGTTTCCATTTCCCT
R—GGAAGGGTTCTGCATGTGTT
Chr4:328582–328860
SSR03 F—GGGCCTGTGATCCATAGAGTAAAA
R- GATGCATGCTTAAATTCTTATTTCTTCA
Chr4:1572774–1572919
SSR04 F—GGATGGAAAAATTGGAAAGAA
R—TGAGGCGACTGTGGTTCATA
Chr:3199559–3199824
SSR05 F—ATGCTTAGTAGGCGGGTTTC
R—TGCCGGAGATTTTAGACTGT
Chr4:3526134–3526604
SSR06 F—AATGGTTGCGTTGAAGTTCC
R—TTGGCATCCTCTTCTTCTTCA
Chr4:5959998–5960201
HHS Vulnerability Disclosure