Table 2. Information on the major markers in chromosome 4 used to analyze the polymorphisms.
Marker Number | Primer Sequence | position | Digest(s) |
---|---|---|---|
CAPS01 | F—AGTTCAATGACCAAAGGTGAACT R—AATGTCACGTTGTCATGTCCA |
Chr4:59846 | BglII(a/gatct):445+206,651 |
CAPS02 | F -TGCAACTACAGGTGTAAGGCA R—CCAACAACCATTCACGCAGG |
Chr4:67981 | SalI(g/tcgac):445+317,762 |
CAPS03 | F—ACCTTGGGAGCCCTTAGAGA R—AGGTAGAACACTCAAACTTAGGGT |
Chr4:137311 | HapII(c/cgg):335+316,651 |
CAPS04 | F—GAACGTCCCAAGGAACCACA R—TCCAACTTCAAACCCTTCCTCT |
Chr4:840557 | TaqI(t/cga):372+352,724 |
CAPS05 | F—CGTACGTATACCACCACCCG R—TGGTCATCGTCATCGCTAAGG |
Chr4:909302 | EcoRI(g/aattc):405+353,758 |
CAPS06 |
F—TGACCTATTCTCGCTTGCCC R—TCCCCTTGCATTTTCCCTCG |
Chr4:999594 | Eco32I(gat/atc):437+292,729 |
CAPS07 | F—AATAGCTCTTCGTTCATGTACTCT R—AGACATCACGTGGAAATTAGAAG |
Chrr4:3362878 | HapII(c/cgg):330+328,658 |
CAPS08 | F—ACCCTACCATGTCTATTAACCATCC R—AGGAAACAATTGTATAGCTATGGTTT |
Chr4:3848297 | HindIII(a/agctt):471+252,723 |
CAPS09 | F—AGTGTTCAAATTAACCAAGGGGA R—ACTCGCATGTCACCTACACG |
Chr4:3874492 | HindIII(a/agctt):458+252,710 |
CAPS10 | F—TGACTACCAATCAGACAAATCTTGA R—GGTGGTCAGTGGGAATTGCA |
Chr4:4156897 | TaqI(t/cga):366+312,678 |
CAPS11 | F—ACATCATCTCTCCCGCTCCA R—TGTTGCGTTTAGGGTTGGTT |
Chr4:4994766 | AluI(ag/ct):473+179,652 |
CAPS12 | F—ACCGCTAAAAACTAAATGAGTGTT R—AGCATGCAAAATAACGCCCG |
Chr4:5533196 | AsuII(tt/cgaa):714,357+357 |
CAPS13 | F—ATCAATTATGCCTCAATAGGTTTCTT R—TGGAGTATTGCATTGAGTAGCA |
Chr4:6486343 | BfaI(c/tag):494+186,680 |
CAPS14 | F—TTCCTTGCAGCCAACTCACT R—CAAGGGTGCTCGAGAAGGTT |
Chr4:6838095 | EcoRI(g/aattc):782,492+290 |
CAPS15 | F—TCCTTCAACAGTTGGCGACT R—CGCCTTGAGTGGGCTATAGG |
Chr4:6862096 | AluI(ag/ct):408+281,689 |
SNP02 | F—TGCATGATGAGCCTTCTTTGAA R—TAGACGGGGCTCACAAGTCA |
Chr4:48456 | |
SNP03 | F—AGAATCAACATGAGAATGCAACA R—ATCAAAGGACGAGCCCTCAC |
Chr4:37932 | |
SSR01 | F—GCATATCTAATCTATGAGCACCTAC R—ACAATATTTTGTTGTAGAGTAGAGGA |
Chr4:74647–75150 | |
SSR02 | F—GGGTTTGTTTCCATTTCCCT R—GGAAGGGTTCTGCATGTGTT |
Chr4:328582–328860 | |
SSR03 | F—GGGCCTGTGATCCATAGAGTAAAA R- GATGCATGCTTAAATTCTTATTTCTTCA |
Chr4:1572774–1572919 | |
SSR04 | F—GGATGGAAAAATTGGAAAGAA R—TGAGGCGACTGTGGTTCATA |
Chr:3199559–3199824 | |
SSR05 | F—ATGCTTAGTAGGCGGGTTTC R—TGCCGGAGATTTTAGACTGT |
Chr4:3526134–3526604 | |
SSR06 | F—AATGGTTGCGTTGAAGTTCC R—TTGGCATCCTCTTCTTCTTCA |
Chr4:5959998–5960201 |