Fig 4. Infection of lipin1-deficient cells with HCV_tcp.

Huh-7 cells were transduced with lentiviral vectors expressing control or LPIN1-specific shRNAs. shLPIN1-1 and shLPIN1-2 target both isoforms, while shLPIN1-3 is specific for lipin1 isoform alpha and shLPIN-4 for isoform beta. Silencing was verified at day 7 post-transduction and control and silenced cells were inoculated with HCV_tcp. Luciferase activity was determined in the different cell lines 48 hours post-inoculation. (A) Total lipin1 protein quantitation by Western-Blot. (B) Relative HCV_tcp infection efficiency. (C) Plot displaying lipin1 expression data versus HCV_tcp infection efficiency and the correlation index adjusted to an exponential curve. Data are shown as average and SD of four independent experiments performed in triplicate (n = 12). Statistical significance was determined using Student´s t-test (*p<0.05; **p<0.01).