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. 2018 Sep 28;13(9):e0204947. doi: 10.1371/journal.pone.0204947

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© 2018 Fullwood et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — BJAB ΔvIL6 cells from culture and single cell suspensions from solid tumors were stained for common B cell markers and analyzed for flow cytometry. B cell markers were analyzed from these different cellular populations to determine if changes in gene expression were evident during the process of tumor development. Populations were gated on the human CD45 marker as indicated by brackets, then various B cell markers were analyzed. (A) Representative histograms of some B cell markers that differed in cultured cells vs tumor cells. (B) Summary of mean populations that were positive for the given marker. (C) Summary of mean fluorescence intensities. n = 5 WT; n = 6 ΔvIL-6. Standard errors are indicated; *, p≤0.05 **, p≤0.01.