Table 1. Analysis of tumors for vIL-6 mRNA expression and measurement of angiogenesis in tumors.
| Virus strain | Mouse | Tumors detected | # tumors analyzed | vIL-6 mRNA detection | Blood vessels/mm2 |
|---|---|---|---|---|---|
| KSHV WT | A | 0 | 0 | n/a | n/a |
| B | 2 | 2 | yes | 0 | |
| C | 7 | 5 | no | 0 | |
| D | 5 | 4 | yes | 0.767 | |
| E | 8 | 3 | yes | 0.018 | |
| F | 4 | 2 | yes | 0 | |
| KSHV ΔvIL-6 | A | 4 | 3 | no | 0.108 |
| B | 2 | 1 | no | 0.945 | |
| C | 1 | 1 | no | 0.432 | |
| D | 3 | 3 | no | 0.635 | |
| E | 4 | 3 | no | 0.552 | |
| F | 2 | 2 | no | 0.015 |
Tumors from 5 WT and 6 ΔvIL-6 mice were prepared for histology by freezing in OCT compound and stored at -80°C, then sectioned to a thickness of 12μm. RNA was extracted from 5 slides and subjected to RT-PCR using a primer set for the vIL-6 mRNA. Other sections were stained with hematoxylin and eosin and analyzed for the presence of blood vessels, which were then correlated to the area of the tumor.