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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Curr Opin Biomed Eng. 2018 Mar 26;6:42–50. doi: 10.1016/j.cobme.2018.03.004

Figure 1.

Figure 1

U87 glioblastoma cell morphology in soft and stiff PEG hydrogels [19]. (A) Cells in soft hydrogels spread starting on day 3 and form extended networks of protrusions over 14 days, while cells in stiff hydrogels spread starting on day 7 and have protrusions that get longer over the culture period. Scale bar = 50 µm. (B) H&E staining and actin (red) and nuclei (blue) staining on soft and stiff hydrogels on day 21 show that cells in soft hydrogels are fibroblast-like and cell spheroids in stiff hydrogels have actin-rich protrusions at the periphery. Scale bar = 75 µm. Reprinted with permission from [19]. Copyright 2014 American Chemical Society.