LplT substrates LPE, LPG, diacyl-CL and deacylated CL (CL-hg) are transported by LplT from the periplasm to the inner leaflet of the IM by an energy-independent mechanism. On the cytoplasmic surface, bifunctional Aas catalyzes acyl transfer to a flipped LPL using acyl-ACP as acyl donor, generating PE, PG or triacyl-CL respectively. Aas also catalyzes ATP-dependent synthesis of acyl-ACP using a fatty acid. LPA, LPC and glycerol-3-phosphate (G3P) are not LplT substrates.