Table 2.

Extrapolation of in vitro intrinsic clearance by isolated hepatocytes to hepatic clearance by isolated perfused livers under different assumptions with respect to chemical binding

Chemical	BSA protein (g/L)	fu,MEAS= fu,PERF/ fu, HEP (unitless)	CLipl,pred,hep; fu = fu,MEAS (mL/h/g liver)	CLipl,pred,hep; fu = 1.0 (mL/h/g liver)	CLipl,meas (mL/h/g liver)
Naphthalene	1	0.20	1.8 ± 0.8	8.5 ± 3.7	8.3 ± 0.1
	10	0.02	0.17 ± 0.08		2.7 ± 0.2
Fluorene	1	0.51	38.8 ± 7.6	52.5 ± 7.2	41.8 ± 10.9
	10	0.12	15.1 ± 4.6		26.2 ± 9.1
Anthracene	1	2.91	64.1 ± 3.4	50.3 ± 5.7	66.6 ± 13.3
	10	0.58	41 ± 6.3		48 ± 7.6
phenanthrene	1	1.69	53.8 ± 5.7	44.1 ± 6.2	61.9 ± 15.2
	10	0.28	21 ± 4.4		43.4 ± 11.7
pyrene	1	1.20	78.4 ± 1.4	82.1 ± 0.02	62.7 ± 2.9
	10	0.25	67.0 ± 4.6		68.2 ± 3.3
Benzo[a]pyrene	1	20.31	69.2 ± 0.2	54.8 ± 3.1	58 ± 13
	10	1.94	61.7 ± 1.7		50.6 ± 4.2

BSA = bovine serum albumin; fu,meas = free fraction in the liver perfusate (fu,PERF)/free fraction in the hepatocyte suspension (fu,HEp); CLipl,pred,hep = predicted hepatic clearance by perfused livers; CLipl,meas = measured hepatic clearance by perfused livers; all data reported as mean ± SD