Table 1. Statistical significance of different behavioral tasks in SHAM and Naive animals.
Prenatal treatment from GD 5 to GD 20 with vehicle solution in control dams (SHAM group) did not alter sociability (social interaction), anxiety (elevated plus maze), and cognition (temporal order and object recognition memory tasks) in adult rats of either sex. Statistical significance was determined using the unpaired Student’s t-test after outliers were detected and removed from the dataset using Grubbs’ test.
| Behavior | Parameter | Treatment | Sex | n | Mean | SEM | P value (Unpaired t test) |
|---|---|---|---|---|---|---|---|
| Social Interaction | # of contacts | NAIVE | Male | 5 | 216.200 | 28.420 | 0.439 |
| SHAM | 10 | 235.800 | 10.550 | ||||
| NAIVE | Female | 4 | 211.800 | 21.600 | 0.85 | ||
| SHAM | 7 | 215.100 | 5.713 | ||||
| Elevated Plus Maze | % Time Open | NAIVE | Male | 6 | 25.900 | 2.988 | 0.665 |
| SHAM | 7 | 24.270 | 2.245 | ||||
| NAIVE | Female | 9 | 25.530 | 2.464 | 0.359 | ||
| SHAM | 9 | 22.540 | 1.978 | ||||
| % Open Entries | NAIVE | Male | 6 | 27.740 | 3.248 | 0.164 | |
| SHAM | 7 | 33.950 | 2.678 | ||||
| NAIVE | Female | 9 | 33.160 | 2.520 | 0.475 | ||
| SHAM | 9 | 30.230 | 3.111 | ||||
| Temporal order | Discrimination index | NAIVE | Male | 8 | 0.320 | 0.085 | 0.702 |
| SHAM | 7 | 0.366 | 0.078 | ||||
| NAIVE | Female | 8 | 0.258 | 0.132 | 0.896 | ||
| SHAM | 7 | 0.277 | 0.038 | ||||
| Object Recognition | Discrimination Index | NAIVE | Male | 10 | 0.547 | 0.087 | 0.792 |
| SHAM | 8 | 0.580 | 0.086 | ||||
| NAIVE | Female | 7 | 0.323 | 0.126 | 0.481 | ||
| SHAM | 7 | 0.421 | 0.045 |