. 2018 Sep 11;7:e36234. doi: 10.7554/eLife.36234

Table 2. Statistical significance of gene expression changes in adult rat medial prefrontal cortex after in utero WIN treatment.

The levels of mRNA’s of interest were determined from medical prefrontal cortex punches using qPCR (See Materials and methods for details). Statistical significance was determined using the unpaired Student’s t-test after outliers were detected and removed from the dataset using Grubbs’ test. p-Values of less than 0.05 are identified by bold text in the table. Relative expression levels for CB1, TRPV1, mGluR5 and DAGL-alpha are shown in Figure 10.

	MALE			Female
Targets	Treatment	N	p-Value (Unpaired t-test)	Treatment	N	p-Value (Unpaired t-test)
CB1	SHAM	8	0.3279	SHAM	7	0.0643
CB1	WIN	8	0.3279	WIN	8	0.0643
CB2	SHAM	7	0.9379	SHAM	6	0.1427
CB2	WIN	7	0.9379	WIN	7	0.1427
TRPV1	SHAM	7	0.2281	SHAM	8	0.0098
TRPV1	WIN	8	0.2281	WIN	7	0.0098
mGlu5	SHAM	7	0.0273	SHAM	8	0.0092
mGlu5	WIN	7	0.0273	WIN	7	0.0092
mGlu1	SHAM	8	0.0400	SHAM	7	0.3910
mGlu1	WIN	8	0.0400	WIN	8	0.3910
DAGL alpha	SHAM	8	0.4370	SHAM	8	0.0024
DAGL alpha	WIN	8	0.4370	WIN	8	0.0024
DAGL beta	SHAM	7	0.4218	SHAM	6	0.3783
DAGL beta	WIN	7	0.4218	WIN	7	0.3783
NAPE-PLD	SHAM	7	0.4258	SHAM	7	0.7518
NAPE-PLD	WIN	7	0.4258	WIN	7	0.7518
MAGL	SHAM	8	0.5097	SHAM	8	0.2147
MAGL	WIN	8	0.5097	WIN	8	0.2147
FAAH	SHAM	8	0.5505	SHAM	8	0.4096
FAAH	WIN	8	0.5505	WIN	7	0.4096
ABHD6	SHAM	7	0.2437	SHAM	6	0.6014
ABHD6	WIN	7	0.2437	WIN	7	0.6014
CRIP1a	SHAM	7	0.6414	SHAM	7	0.1887
CRIP1a	WIN	7	0.6414	WIN	7	0.1887