Fig. 2.

Cicaprost decreased the numbers of IL-4⁺CD4⁺, IL-5⁺CD4⁺ and IL-13⁺CD4⁺ T cells. Naïve CD4⁺ T cells from WT and IP KO mice were stimulated with anti-CD3 and anti-CD28 Abs in the absence or presence of IL-33 and treated with vehicle or cicaprost for 3 days. The cells were then stimulated with PMA and ionomycin in the presence of GolgiStop for 4h. The levels of intracellular cytokines were determined by flow cytometry. A. Flow gating strategy. The cells were gated for lymphocytes, single cells, and live cells and analyzed for IL-4, IL-5, and IL-13 expression in CD4⁺ cells. B-D. The number of IL-4⁺CD4⁺ (B), IL-5⁺CD4⁺ (C) and IL-13⁺CD4⁺ (D) T cells. Data are combined of 2 experiments and presented as mean ± SEM. *, p < 0.05, n=6.