Fig. 4.

Cicaprost decreased IL-2 production and NFAT activation in CD4⁺ T cells. Naïve CD4⁺ T cells from WT and IP KO mice were stimulated with anti-CD3 and anti-CD28 Abs in the absence or presence of IL-33 and were treated with vehicle or cicaprost. A. The levels of IL-2 in the culture supernatant at day 2 were determined by ELISA. B. Cells were transduced without or with NFAT-luciferase reporter lentiviral vector. Cells were harvested on day 3 for luciferase assay. Data are combined of 3 (A) and 2 (B) experiments and presented as mean ± SEM. *, p < 0.05, n=9 (A) and 6 (B).