Table 1.

Relative gene expression. Gene expression was run in duplicate and analyzed with the 2^ΔΔCt method relative to β-actin.

Group	Adult				Aged
	Vehicle		M. vaccae		Vehicle		M. vaccae
	Sham	Laparotomy	Sham	Laparotomy	Sham	Laparotomy	Sham	Laparotomy
Gene	Three days post-laparotomy (behaviorally naïve rats)
Arg1	1.00 ± 0.08	1.06 ± 0.07	1.09 ± 0.08	1.16 ± 0.11	0.94 ± 0.07	0.9 ± 0.07	1.26 ± 0.07	1.26 ± 0.05
CD200L	1.00 ± 0.08	1.02 ± 0.07	0.98 ± 0.06	0.99 ± 0.11	0.82 ± 0.06	0.79 ± 0.05	0.94 ± 0.06	1.03 ± 0.12
CD206	1.00 ± 0.09	0.81 ± 0.09	0.88 ± 0.15	0.94 ± 0.11	0.59 ± 0.04	0.49 ± 0.06	0.69 ± 0.04	0.75 ± 0.08
CD-4	1.00 ± 0.07	0.85 ± 0.05	0.92 ± 0.07	0.82 ± 0.07	2.00 ± 0.14	1.80 ± 0.17	1.8 ± 0.14	1.80 ± 0.05
IL-4	1.00 ± 0.10	0.93 ± 0.16	0.94 ± 0.15	0.88 ± 0.10	0.81 ± 0.07	0.56 ± 0.04	1.05 ± 0.17	1.10 ± 0.10
IL-10	1.00 ± 0.17	0.79 ± 0.16	1.02 ± 0.19	0.96 ± 0.31	0.50 ± 0.15	0.43 ± 0.11	0.70 ± 0.13	0.62 ± 0.23
IL-1β	1.00 ± 0.12	0.90 ± 0.07	0.97 ± 0.08	0.83 ± 0.09	0.95 ± 0.08	1.44 ± 0.15	0.83 ± 0.13	0.84 ± 0.16
Foxp3	1.00 ± 0.10	1.09 ± 0.10	1.18 ± 0.12	1.16 ± 0.09	1.14 ± 0.14	1.11 ± 0.09	1.52 ± 0.23	1.55 ± 0.19
NFKBIA	1.00 ± 0.08	1.10 ± 0.10	0.96 ± 0.07	1.05 ± 0.09	1.21 ± 0.06	1.62 ± 0.15	1.05 ± 0.07	1.28 ± 0.17
MHCII	1.00 ± 0.12	0.73 ± 0.08	0.95 ± 0.14	0.73 ± 0.07	4.41 ± 0.29	3.98 ± 0.48	2.94 ± 0.19	2.84 ± 0.37
TGF-β	1.00 ± 0.08	0.84 ± 0.06	0.94 ± 0.06	0.82 ± 0.14	1.04 ± 0.04	0.93 ± 0.06	0.89 ± 0.05	0.97 ± 0.05
Eight days post-laparotomy (behavior rats)
Arg1	1.00 ± 0.06	0.81 ± 0.09	0.90 ± 0.11	0.80 ± 0.09	0.71 ± 0.08	0.69 ± 0.06	0.90 ± 0.07	1.07 ± 0.07
IL-4	1.00 ± 0.17	1.02 ± 0.07	1.19 ± 0.14	1.06 ± 0.10	0.71 ± 0.09	0.61 ± 0.10	1.06 ± 0.11	1.04 ± 0.07
IL-1β	1.00 ± 0.14	1.19 ± 0.09	1.07 ± 0.08	1.10 ± 0.05	1.11 ± 0.14	0.95 ± 0.13	1.13 ± 0.14	0.87 ± 0.09