Skip to main content
. Author manuscript; available in PMC: 2018 Dec 6.
Published in final edited form as: Mucosal Immunol. 2018 Jun 6;11(5):1316–1328. doi: 10.1038/s41385-018-0034-3

Figure 7. Neonatal p40 treatment prevents colitis in adult mice.

Figure 7

Mice were treated with p40 from postnatal day 2 to day 21, as described in Figure 2. Colitis was induced in 7 week old mice by TNBS in ethanol intrarectally (a–c) and by 3% DSS in drinking water for 4 days (d–f). Mice receiving ethanol and water were used as controls for TNBS and DSS treatment, respectively. Mice were euthanized four days after TNBS treatment and at the fourth day of DSS treatment. (a,b and d,e) Colon sections were stained with H&E for light microscopic assessment of inflammation. The inflammation/injury scores are shown. (c and g) mRNA was isolated from the colonic tissues for real-time PCR analysis of indicated cytokine mRNA expression levels. The average of cytokine mRNA expression levels in the control mice of the no-p40 group was set as 100%, and the mRNA expression level of each mouse was compared to this average. (f) MPO activity in the colonic tissue lysates was detected. *p<0.05 compared to the control mice in the no-p40 group. # p<0.05 compared to the p40 group with TNBS or DSS treatment. n=5 in control groups, n=7 in TNBS- and DSS-treated groups.