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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Water Res. 2018 Jun 30;144:1–12. doi: 10.1016/j.watres.2018.06.066

Table 1.

The reverse transcription-PCR and qPCR assays most commonly used to detect PMMoV in (waste)water and food matrices.

Assay type Oligonucleotide name Sequence (5’ – 3’) Target gene Amplicon size (bp) Thermocycler
conditions
 Citation
PCR PMMV-F AACCTTTCCAGCACTGCG Replication-
associated
protein
201 94°C 5 min, 40x
(94°C 1 min,
50°C 45 s, 72°C
1 min), 72°C 5
min
Zhang et al. 2006
PMMV-R GCGCCTATGTCGTCAAGACT
PCR PMMoV-CP forward ATGGCTTACACAGTTTCCAGT Capsid
protein
474 94°C 3 min, 40x
(94°C 30 s,
55°C 30 s,
72°C 30 s)
Peng et al. 2015
PMMoV-CP reverse CTAAGGAGTTGTAGCCCAGGTG
PCR CP/s ATGGCATACACAGTTACCAGT Capsid
protein
470 94°C 2 min, 40x
(94°C 1 min,
49°C 45 s,
72°C
1 min), 72°C 7 min
Çağlar et al. 2013
CP/a TTAAGGAGTTGTAGCCCACGTA
qPCR
(SYBR
green)
Ha-PMMV1 GTGGCAGCAAAGGTAATGGT Replication-
associated
protein
80 95°C 15 min, 45x
(58°C 45 s,
72°C 15 s)
Hamza et al. 2011
Ha-PMMV2 ATTTGCTTCGGTAGGCCTCT
PM1602 TGTTTCGGAAAAGGCTCTTG
qPCR
(hydrolysis
probe)
PMMV-FP1 GAGTGGTTTGACCTTAACGTTGA Replication-
associated
protein
68 95°C 10 min,
40x (95°C 30 s,
60°C 1 min)
Zhang et al. 2006
PMMV-RP1 TTGTCGGTTGCAATGCAAGT
PMMV-probe1 6FAM-CCTACCGAAGCAAATG-TAMRA
PMMV-FP1-rev GAGTGGTTTGACCTTAACGTTTGA Haramoto et al. 2013
PMMV- Taqman MGB probe 6FAM-CCTACCGAAGCAAATG-MGB-NFQ