Skip to main content
. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Semin Cell Dev Biol. 2018 Mar 28;86:92–101. doi: 10.1016/j.semcdb.2018.03.018

Figure 4.

Figure 4

The T7 Replisome. The hexameric helicase/primase unwinds the DNA duplex to provide the template for DNA synthesis. The leading strand is continuously extended by T7 DNA polymerase in complex with its processivity factor, E. coli Trx, in association with the helicase. The primase synthesizes RNA primers on the lagging strand that are then extended by T7 DNA polymerase/thioredoxin leading to the formation of a replication loop containing a nascent Okazaki fragment. The T7 ssDNA-binding protein, gp2.5, stabilizes ssDNA replication intermediates, interacts with T7 DNA polymerase and helicase/primase, and is essential for the coordination of DNA synthesis on both strands.