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. 2018 Sep 28;8:14502. doi: 10.1038/s41598-018-32947-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dose-dependent anti-cancer activity of PE-BBI, ranging from 1 to 100 µM, against different human colorectal cancer cell lines (A) DKS8 (B) DLD-1 (C) HKE3 (D) HCT116 (E) FHC. The IC₅₀ values against four cancer cell lines after 24 h incubation, were 50.1 µM, 9.8 µM, 35.4 µM, 50.2 µM, respectively. Meanwhile, PE-BBI didn’t show any cytotoxicity against human normal endothelial cell lines FHC. Data points in each panel represent the mean and SD of values obtained for 9 replicates. Significant differences were marked with asterisks (*P < 0.05; **P < 0,01; ***P < 0.001).