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. 2018 Sep 28;8:14527. doi: 10.1038/s41598-018-32824-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Live cell imaging of SiR-labelled 150BCNK-EGFP-CAAX expressed in COS7 cells. Top row: red SiR; centre row: green EGFP; bottom row: merge. The scale bar represents 5 μm. (B) Line intensity profiles of 488 and 640 nm channels (EGFP and SiR, respectively) are presented as a percentage of the maximum value and are plotted as a function of distance along a line (yellow line in top panel A). (C) Live-cell imaging of SiR-labelled 45BCNK-α-tubulin expressed in COS7 cells. Zoomed-in images of a subset of the cell are presented below. The scale bar represents 5 μm. (D) Line intensity profiles of 640 nm channel presented as a percentage of the maximum value and plotted as a function of distance along a line (yellow line in top panel C). (E) Signal-to-noise ratios calculated from live-cell images of SiR-labelled 150BCNK-EGFP-CAAX and 45BCNK-α-tubulin expressed in COS7 cells using plasmids carrying 1 or 4 copies of PylT (±SEM). The green dashed line marks the signal-to-noise ratio of GFP-labelled α-tubulin visualized in live COS7 cells under identical conditions.