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. 2018 Sep 28;8:14558. doi: 10.1038/s41598-018-32347-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The histogram of the log of the necrosis rate, $λ_{V N}$ , for four FBS concentrations of 7.5%, 5.0%, 2.5%, and 0.0%, from top to bottom, respectively, and three initial conditions, 25,000, 50,000, and 100,000 cells/ml, from right to left, respectively. The necrosis rate increases as the FBS concentration decreases. The panels presenting 2.5%, 5.0%, and 7.5% FBS indicate that there is little to no necrosis for these cases. It is only when the FBS level drops to zero, that evidence of necrosis appears.