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. 2018 Sep 28;8:14543. doi: 10.1038/s41598-018-32400-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Infection of differentiated SH-SY5Y cells with ZIKV strains representing African and multiple Asian lineages. Viral infection is monitored by immunofluorescent staining of differentiated SH-SY5Y cells at 48 hours post-infection using the anti-Env monoclonal antibody (green). Cells are treated with 2 days of retinoic acid prior to ZIKV infection. The images shown are representative images taken from 3 independent experiments, with observation of a minimum of 10 fields under both low (10X) and high (40X and 63X) magnification per experiment. The scale bar represents 50 µm.