Table 1.
Neuronal markers expressed in differentiated SH-SY5Y cells.
| Marker | log2 fold change (p-value)a | IFA signal, undifferentiatedc | IFA signal (RA-differentiated)b,c |
predicted effect after differentiation |
|---|---|---|---|---|
| Synaptophysin | 1.96 (4.57E-14) | + | +++ | up-regulation |
| MAP2 | 1.37 (2.14E-05) | + | +++ | up-regulation |
| ID2 | −0.13 (3.68E-86) | ND | ND | down-regulation |
| vimentin | −0.75 (1.01E-03) | +++ | + | down-regulation |
| SOX2 | NSC | − | − | down-regulation |
| GFAP | NSC | − | − | no effect |
| SatB | ND | − | +++ | up-regulation |
| TH | ND | − | +++ | up-regulation |
aRA-differentiated versus undifferentiated cells; based on 3 independent replicates.
bcells were differentiated with 48 hours of RA treatment.
c“−”, no fluorescent signal detected in any cell; “+”, low signal detected in <20% of cells; “+++”, strong signal detected in >70% of cells.
Abbreviations: RA, retinoic acid; SD, standard deviation; IFA, immunofluorescence assay; NEG, negative; POS, positive; MAP2, microtubule-associated protein-2; ID2, inhibitor of DNA binding 2; SOX2, SRY (sex determining region Y)-box 2; GFAP, Glial fibrillary acidic protein; SatB, Special AT-Rich Sequence Binding Protein; and TH, tyrosine hydroxyolase; NSC, not significant change; ND, test not done.