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. 2018 Sep 28;8:14540. doi: 10.1038/s41598-018-32752-0

Figure 5.

Figure 5

(A) The simultaneous changes in prefrontal Oxy-Hb (NIRS-channel 5), forehead and cheek skin blood flow, hand skin blood flow, and MAP during positively-charged comedy stimulation in 18 subjects. The tracings of the averaged time course data at the three trials of comedy stimulation are superimposed. (B) using the average time course data, the Spearman correlation analysis was conducted between the changes in skin blood flow (cheek, forehead, or hand) or MAP and the Oxy-Hb response obtained in a given NIRS channel. The square values (ϒ2) of the Spearman correlation coefficients are plotted against the NIRS channel number. The data (ϒ2) were statistically analysed by two way repeated measures ANOVA, having the main effect of the peripheral variables (skin blood flow and MAP) and prefrontal location. #, Significant difference (P < 0.05) among the peripheral variables (two way repeated measures ANOVA and posthoc Student-Newman-Keuls test). About forehead skin blood flow, the channels of 1, 4, 5, and 9 [indicated by blue arrows (↓)] had greater ϒ2 (P < 0.05) than the channels of 11, 12, 15–17, and 19–21; about cheek skin blood flow, the channels of 1, 4, and 5 [indicated by black arrows (↓)] had greater ϒ2 (P < 0.05) than the channels of 12, 15–17, and 19–21.