Figure 4.
Cells Carrying Premature Stop Codons Fail to Accumulate in the LZ
(A) The frequency of cells carrying Ighv region premature stop codons in SWHELxFucci2 LZ and DZ populations was determined. Plot and gate are from a representative mouse, numbers indicating means (+/− SEM) from five independent experiments.
(B) The phenotype of LZ cells with premature stop codons is shown for all three mice. Data are overlaid with total GC B cells that were not sequenced (gray), for reference.
(C) Summary from five independent experiments. Numbers above bars indicate number of each cell type sequenced. Numbers below indicate frequency of detected LZ stop codons that were BCRhigh and have been normalized for where rare populations were enrichments performed during FACS sorting.