REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-GFP (clones 7.1 and 13.1)	Roche	Cat# 11814460001
Mouse monoclonal anti-FLAG (clone M2)	Sigma-Aldrich	Cat# F-1804
Mouse monoclonal anti-Myc (clone 9B11)	Cell Signalling	Cat# 2276
Mouse monoclonal anti -V5 (clone 2F11F7)	Invitrogen	Cat# 37-7500
Mouse monoclonal anti-actin (clone AC-40)	Sigma-Aldrich	Cat# A3853
Rat monoclonal anti-HA (clone 3F10)	Roche	Cat# 11 867 423 001
Mouse monoclonal anti-Erk1/2 (clone L34F12)	Cell Signalling	Cat# 4696
Anti-phosphoErk1/2 (clone D13.14.4E)	Cell Signalling	Cat# 4370
Rabbit polyclonal anti-PSPH	ThermoFisher	Cat# PA5-22003
Rabbit polyclonal anti-PSAT	Abcam	Cat# ab96136
Bacterial and Virus Strains
E. coli BL21(DE3)	New England BioLabs	Cat# C2527
NEB Stable	New England BioLabs	Cat# C3040
Chemicals, Peptides, and Recombinant Proteins
2 (DL-AP4)	Abcam	Cat# ab120001
Critical Commercial Assays
AMP-Glo	Promega	Cat# V5011
CellTiter-Glo 2.0	Promega	Cat# G9241
Experimental Models: Cell Lines
HEK293	ATCC	Cat# CRL-1573
Oligonucleotides
Biotinylated northern blot probe: AAC CCA CGT AAG GCA ATT TTA GAG ACT GCT GCC TAA CCC CT	Integrated DNA Technologies	N/A
Fluorescent northern blot probe (quantitative): /5IDR800/GTG GGA TTT GAA CCC ACG TAA GGC AAT TTT	Integrated DNA Technologies	N/A
Guide RNA targeting PSPH exon 2 Top: CACCGCACACCTGACCCCCGGCATA Bottom: AAACTATGCCGGGGGTCAGGTGTGC	Integrated DNA Technologies	N/A
Guide RNA targeting PSAT exon 1 Top: CACCGCCAGGCAGGTGGTCAACTTT Bottom: AAACAAAGTTGACCACCTGCCTGGC	Integrated DNA Technologies	N/A
Recombinant DNA
pET20b	Novagen	Cat# 69739-3
pcDNA3.4	ThermoFisher	Cat# A14697
(U6-PylT*)4/EF1α-PylRS	Schmied et al., 2014	N/A
Software and Algorithms
Prism 7	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Other
GFP-Trap MA beads	ChromoTek	Cat# gtma-20