Figure 1.

Intracellular ROS of HOS cells exposed to DHPs and H₂O₂.Cells were treated with DHPs and hydrogen peroxide, without the exchange of culture medium, as follows: (A,B) 1-h DHPs + hydrogen peroxide, or (C,D) 1-h hydrogen peroxide + DHPs. The fluorescence reflecting intracellular ROS levels was measured at different time points (0—no treatment, 5—immediately after the first treatment, 30, 60, 65—immediately after the second treatment, 90, 120, 180, 240 min). Results of DHPs (A,C—water-soluble; B,D—soluble in organic solvents) are presented as mean of relative fluorescence units (RFU) ± standard deviation (n = 2). Controls: ctrl (untreated cells), ctrl H₂O₂ (cells treated with hydrogen peroxide) and solvent-controls (vehicle Diethone I and vehicle E3-46 IV-3).