Table 2.
Activity of Akt1 variants.
| Akt1 Variant | Akt1 Amount (pmol) | Initial Velocity vo (fmol/min) | Apparent Catalytic Rate kapp (fmol/min/pmol Akt1) | Activation (Fold Increase) | Reference |
|---|---|---|---|---|---|
| Akt1 (unphosphorylated) | 18 | 0.6 ± 0.2 | 0.03 ± 0.01 | 1.0 ± 0.3 | [12] |
| pAkt1S473 | 18 | 46 ± 5 | 2.6 ± 0.3 | 85 ± 9 | [12] |
| pAkt1T308 | 1.8 | 22 ± 4 | 12 ± 2 | 400 ± 70 | [12] |
| ppAkt1T308,S473 | 1.8 | 79 ± 11 | 44 ± 6 | 1500 ± 200 | [12] |
| ΔPHAkt1 (unphosphorylated) | 18 | 1.4 ± 0.1 | 0.079 ± 0.006 | 2.7 ± 0.2 | this study |
| ΔPH pAkt1S473 | 18 | 210 ± 20 | 12 ± 1 | 390 ± 40 | this study |
| ΔPH pAkt1T308 | 0.18 | 370 ± 100 | 2100 ± 600 | 6900 ± 1800 | this study |
| ΔPH ppAkt1T308,S473 | 0.18 | 2300 ± 600 | (13 ± 3) × 103 | (4 ± 1) × 105 | this study |
| Commercial Akt1 (Abcam) | |||||
| Lot 1 | 18 | 100 ± 20 | 6 ± 1 | 200 ± 30 | this study |
| Lot 2 | 18 | 2200 ± 700 | 120 ± 40 | 4000 ± 1000 | this study |